Prostaglandin E1 (PGE1) Rapid ELISA kit

This Rapid ELISA kit applies Competitive Inhibition enzyme immunoassay technique for the quantitative detection. The micro ELISA plate provided in this kit has been pre-coated with PGE1. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to PGE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Catalog No.3510002
Unit Size 96-Well Tests
Product CategoryRapid ELISA
Reactivity Various
Sample Cell Culture Supernatant; Plasma; Saliva; Serum; Urine
Assay Method Competitive Inhibition ELISA
Assay Duration 45 min
Sensitivity 9.55 pg/mL
Standard Curve Range 31.25-2000 pg/mL
Storage/Stability -20°C/1 year; 4°C/6 months

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