Nissl stain kit (methylene blue method)

Nissl body is a triangular or oval small block substance distributed in the cytoplasm of nerve cells, which can be stained by basic dyes such as thionine, methylene blue, toluidine blue and tar violet. Such dyes are dyed purple-blue. The main advantages of Nissl stain solution (Nissl Stain, methylene blue method) are easy operation, stable staining, and wide application range. It can be used for staining Nissl substances and neurons in paraffin tissue sections, and the presence and disappearance of Nissl bodies. It is an important indicator of whether nerve cells are damaged. When encephalitis, cerebral ischemia, axonal reaction, etc. occur, Nissl will dissolve or even disappear.