McCoy's 5A (with L-glutamine, Phenol red; w/o Sodium Pyruvate, HEPES)

McCoy's 5A modified medium is a general-purpose medium, originally designed by Thomas McCoy and others specifically for Novikoff Hepatoma cells. Compared with other media, McCoy's 5A modified contains high concentrations of D-glucose, bacterial peptone, and reduced glutathione. McCoy's 5A modified is suitable for the cultivation of many types of primary cells, such as cells from bone marrow, skin, kidney, spleen, lung, rat embryo, omentum and other tissues. In addition, it is also used for tissue biopsy culture, cell line establishment, and some lymphocytes and cells that are difficult to culture.