Lipopolysaccharides (LPS) Rapid ELISA kit

This Rapid ELISA kit applies Competitive Inhibition enzyme immunoassay technique for the quantitative detection. The micro ELISA plate provided in this kit has been pre-coated with LPS. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to LPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LPS in the samples is then determined by comparing the OD of the samples to the standard curve.
Catalog No.3511658
Unit Size 96-Well Tests
Product CategoryRapid ELISA
Reactivity Various
Sample Serum; Plasma; Tissue Homogenate; Other Biological Samples
Assay Method Competitive Inhibition ELISA
Assay Duration 45 min
Sensitivity 0.94 ng/mL
Standard Curve Range 1.57-100 ng/mL
Storage/Stability -20°C/1 year; 4°C/6 months

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