Human Adrenocorticotropic Hormone (ACTH) Rapid ELISA kit

This Rapid ELISA kit applies Competitive Inhibition enzyme immunoassay technique for the quantitative detection. The micro ELISA plate provided in this kit has been pre-coated with Human ACTH. During the reaction, Human ACTH in the sample or standard competes with a fixed amount of Human ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Catalog No.3521070
Unit Size 96-Well Tests
Product CategoryRapid ELISA
Reactivity Human
Sample Serum; Plasma; Tissue Homogenate; Other Biological Samples
Assay Method Competitive Inhibition ELISA
Assay Duration 45 min
Sensitivity 9.38 pg/mL
Standard Curve Range 15.63-1000 pg/mL
Storage/Stability -20°C/1 year; 4°C/6 months

Available Size & Price Options

Select Qty

DescriptionSizeQuantity
No Product in Quote List.