Homocysteine (HCy) Rapid ELISA kit

This Rapid ELISA kit applies Competitive Inhibition enzyme immunoassay technique for the quantitative detection. The micro ELISA plate provided in this kit has been pre-coated with HCy. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to HCy. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HCy in the samples is then determined by comparing the OD of the samples to the standard curve.
Catalog No.3510090
Unit Size 96-Well Tests
Product CategoryRapid ELISA
Reactivity Various
Sample Serum; Plasma; Other Biological Fluids
Assay Method Competitive Inhibition ELISA
Assay Duration 45 min
Sensitivity 39.55 ng/mL
Standard Curve Range 125-8000 ng/mL
Storage/Stability -20°C/1 year; 4°C/6 months

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