Homocysteine (HCy) Rapid ELISA kit
This Rapid ELISA kit applies Competitive Inhibition enzyme immunoassay technique for the quantitative detection. The micro ELISA plate provided in this kit has been pre-coated with HCy. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to HCy. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HCy in the samples is then determined by comparing the OD of the samples to the standard curve.
Catalog No. | 3510090 |
Unit Size | 96-Well Tests |
Product Category | Rapid ELISA |
Reactivity | Various |
Sample | Serum; Plasma; Other Biological Fluids |
Assay Method | Competitive Inhibition ELISA |
Assay Duration | 45 min |
Sensitivity | 39.55 ng/mL |
Standard Curve Range | 125-8000 ng/mL |
Storage/Stability | -20°C/1 year; 4°C/6 months |