Deacetylase Inhibitor [100x]

Bacterial Protease & Phosphatase Inhibitor contains many endogenous proteases, phosphatases, etc., which easily lead to degradation or de-modification of proteins in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate protease, phosphatase and other inhibitors to the extract is to prevent protein degradation. This product is a mixture of protease and phosphatase inhibitors for bacterial protein extraction. It contains a broad spectrum of serine, cysteine and acid protease inhibitors/aminopeptidase inhibitors, and serine/threonine, tyrosine, acid and alkaline phosphatase inhibitors. Such as It is not suitable to add EDTA for detecting metalloproteinase activity. Mix the Protease & Phosphatase inhibitor and 0.05M EDTA are added to the lysis solution, which can be used for bacterial protein extraction and effectively inhibit protein degradation.

Scope of application: inhibit various proteases in bacterial extracts (such as serine proteases, aminopeptidases, cysteine proteases, threonine and aspartame acid proteases, metalloproteases, etc.) and phosphatases (such as serine/threonine, tyrosine, acidic and alkaline phosphatase, etc.). Apply to Western Blot and immunoprecipitation to detect phosphorylated protein, protein kinase activity determination, etc.