Custom Recombinant Antibody

Recombinant Antibodies (rAbs) are monoclonal antibodies, generated in vitro using synthetic genes. Unlike monoclonal antibodies (mAbs) which are produced using traditional hybridoma-based technologies, rAbs do not need hybridomas and animals in the production process.
The technology involves recovering antibody genes from source cells, amplifying and cloning the genes into an appropriate phage vector, introducing the vector into a host (bacteria, yeast, or mammalian cell lines), and achieving expression of adequate amounts of functional antibody.
Recombinant Antibody provide highest level of consistency and unrivaled reproducibility; improved sensitivity and confirmed specificity.

Procedure:
Codon optimization and gene synthesis
Subcloning into an expression vector
Plasmid amplification and preparation
Transfection of mammalian cells
Transient expression and purification
QC analysis

Expression Host: CHO / HEK299 cells
Material Supplied: Purified antibody with COA; Expression plasmid containing GOI
Quality Control: SDS-PAGE >95%; SEC-HPLC detection; Endotoxin Level less then 1EU/mg

Competitive Price
Fast Turnaround Time (only 3-4 weeks)
Guaranteed Amount (up to mg level / no antibody no charge)