Arachidonic Acid (AA) Rapid ELISA kit

This Rapid ELISA kit applies Competitive Inhibition enzyme immunoassay technique for the quantitative detection. The micro ELISA plate provided in this kit has been pre-coated with Arachidonic Acid (AA) protein. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Arachidonic Acid (AA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonic Acid (AA) in the samples is then determined by comparing the OD of the samples to the standard curve.