API5 blocking peptide
Blocking peptide binds specifically to the target antibody and block undesirable antibody binding. This occurs because the peptide resembles the epitope for which the antibody is specific, therefore, the antibody binds to the blocking peptide are no longer able to bind to the epitope in the protein.
Pre-adsorption with blocking peptide greatly improves the results and it is extremely useful in immunohistochemistry (IHC) or in Western blotting (immunobloting) when non-specific binding is an issue.
This peptide is recommended to block anti-API5 Antibody (Catalog no. 4072296) reactivity
Directions for Use: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours
Pre-adsorption with blocking peptide greatly improves the results and it is extremely useful in immunohistochemistry (IHC) or in Western blotting (immunobloting) when non-specific binding is an issue.
This peptide is recommended to block anti-API5 Antibody (Catalog no. 4072296) reactivity
Directions for Use: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4°C for overnight or at room temperature for 2 hours
| Catalog No. | 5072296 |
| Unit Size | 1mg |
| Product Category | Blocking Peptide |
| Gene Symbol | API5 |
| UniProt ID | Q9BZZ5 |
| Purity | >85% |
| Synonyms | Apoptosis inhibitor 5; API-5; Antiapoptosis clone 11 protein; AAC-11; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL |
| Storage/Stability | -20°C/1 year |
| Shipping | Gel Packs |