3-NT (3-Nitrotyrosine) ELISA kit
This kit applies competitive inhibition ELISA method for the quantitative detection of 3-NT (3-Nitrotyrosine) in various species samples. The microtiter plate provided in this kit has been pre-coated with 3-NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
Catalog No. | 3007866 |
Unit Size | 96-Wells |
Product Category | ELISA (Quantitative) |
Reactivity | Various |
Sample | Serum; Plasma; Other Biological Fluids |
Assay Method | Competitive Inhibition ELISA |
Assay Duration | 2 hours |
Sensitivity | 1.35 ng/mL |
Standard Curve Range | 4.69-300 ng/mL |
Storage/Stability | -20°C/1 year; 4°C/6 months |