25-Hydroxyvitamin D3 (HVD3) Rapid ELISA kit

This Rapid ELISA kit applies Competitive Inhibition enzyme immunoassay technique for the quantitative detection. The micro ELISA plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3 (HVD3) protein. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3 (HVD3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-Hydroxyvitamin D3 (HVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.